Paratuberculosis or Johne's disease caused by Mycobacterium avium subspecies paratuberculosis is an infectious disease of ruminants, responsible for huge economic losses in animal production systems and has potential to affect international trade (OIE, 2004). In terms of various production losses (livestock traits) affected by the Johne's disease (JD) include; milk production, body condition, body weights, reproductive efficiency and culling rate of goats. Disease is endemic in goats in the northern region of India. At present country lacks control measures (vaccine) against JD. Endemicity of the MAP infection in Jamunapari farm has resulted in heavy losses due to morbidity, mortality and untimely culling of animals on the basis of Johne's disease and other health problems. So far the efforts to control disease in this farm of high milk yielding goats of Jamunapari breed, on the basis of screening of animals (general herds or suspected goats) by fecal examination / fecal culture and culling has not been successful, instead prevalence of disease continued to increase. Goats were under same system of management since last 5-8 years. Indigenous inactivated vaccine developed using native strain of Mycobacterium avium subspecies paratuberculosis (MAP) 'Bison type' has been used to control disease in the breeding farm of Jamunapari goats (endemic for Johne's disease). Herd-level assessment was performed to check the efficacy of indigenous vaccine containing highly pathogenic field strain of MAP 'Bison type'. In Jammunapari herd 526 goats with high prevalence of JD (significantly high annual morbidity, mortality and culling) were vaccinated with 1 ml of indigenous vaccine subcutaneously, in the 3^rd week of September, 2006 and were monitored for different physical traits (morbidity, mortality, body weight, milk yield, kidding rate, birth weight of born kids) and humoral response. PER and post-vaccination data for same period and season was compared. First visible and significant response was in coat colour, texture, skin luster and regeneration of hair, within one month of vaccination. There was significant improvement in body weights gained in feedlot experiments and general herds, age at first kidding, milk yield at 90 days interval, ready to culled and stunted kids, morbidity, mortality and humoral immune response. There was improvement in birth weights, body weights gained at 3 months, kidding percent and litter size. Indigenous vaccine showed overall improvement in all the production scores and traits and immune response in the goatherd of Jamunapari breed, after vaccination.