| Title |
Epidemiological survey of Mycobacterium avium subspecies paratuberculosis isolates in Europe |
| Author(s) |
Karen Stevenson1,
Linda May1,
Susan Denham1,
Ian Heron1,
Lucia de Juan2,
Julio Alvarez2,
Gerald Friedrich Gerlach3,
Karen Dohmann3,
Ivo Pavlik4,
Marketa Kopecna4,
Peter Willemsen5,
Douwe Bakker5,
Virginie Thibault6,
Franck Biet6,
Alastair Greig7.
|
| Institution(s) |
1 Moredun Research Institute, Pentlands Science Park, Bush Loan, Penicuik EH26 0PZ, Scotland, UK;
2 Dpto. Sanidad Animal, Facultad de Veterinaria, Universidad Complutense, HCV - Planta sótano, Avenida Puerta de Hierro s/n, 28040 Madrid, Spain;
3 Institut für Mikrobiologie und Tierseuchen, Tierärztliche Hochschule Hannover, Bischfsholer Damm 15, 30173 Hannover, Germany;
4 Veterinary Research Institute, Hudcova 70, 621 32 Brno, Czech Republic;
5 Central Institute for Animal Disease Control (CIDC-Lelystad), CIDC-Lelystad, Edelhertweg 15, 8203 AA Lelystad, The Netherlands;
6 UR 1282, Infectiologie Animale, Santé Publique (IASP-311), INRA centre de Tours, F-37380 Nouzilly, France;
7 Scottish Agricultural College, Veterinary Science Division, Cleeve Gardens, Oakbank Road, Perth, Scotland, UK.
|
| Source |
Ninth International Colloquium on Paratuberculosis
|
| Section |
5:
Epidemiology and control strategies
|
| Presentation |
Oral
|
| Abstract |
A panel
was assembled of 168 field isolates of Mycobacterium
avium subspecies paratuberculosis isolated from 19
different host species from the Czech Republic, Finland, Germany,
Greece, The Netherlands, Norway, Spain and the United Kingdom. The
panel was typed by pulsed-field gel electrophoresis (PFGE),
restriction fragment length polymorphism and hybridisation to
IS900 (RFLP-IS900), mycobacterial interspersed
repetitive repeats and variable number tandem repeat (MIRU-VNTR)
and amplified fragment length polymorphism. A total of 17 BstEII
profiles were detected by RFLP-IS900 analysis and the C1
profile was found to be the most predominant in Europe. Thirty one
different multiplex PFGE profiles were detected using SnaBI and
SpeI and the most widely distributed profile was [2-1]. Twenty five
different MIRU-VNTR types were detected with INMV1 and 2 being the
most widely disseminated. A few strains were found to be restricted
to specific geographic locations although larger numbers are
required to determine if this is significant. No evidence was found
for species-specific strains and where details were available,
wildlife isolates on a single farm were found to be identical to
those of cattle on the same farm suggesting interspecies
transmission. A comparison of the discriminatory power of the
various techniques indicated that PFGE was the most discriminatory
followed by MIRU-VNTR and if both of these techniques were combined
the discriminatory power was sufficient for epidemiological
surveys.
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