Vaginal secretions and serum from 29 post-parturient farm goats were screened by culture, IS900 PCR and indigenous ELISA kit to know the presence of MAP in reproductive tract discharges after parturition. Decontaminated pellets from vaginal secretions and MAP cultures were processed for DNA isolation and screened by IS900 PCR. Positive DNA samples on amplification yielded specific 229 bp band from pellets of vaginal secretions (VS). Of 10 DNA obtained from 29 pellets, 4 were amplified. Soluble protoplasmic (PPA) antigen from MAP 'Bison type' strain cultured from terminal case of JD in goat was used in ELISA kit. The 37.9%, vaginal secretions each were positive in culture and ELISA from 29 goats of farm herds. Of the 3 tests culture was most sensitive. There was poor correlation (37.5%) between culture of VS and ELISA kit (18.1% in strong positives and ELISA and very good correlation between positives (80.0%), low positive and suspected (50.0%) and culture. These results clearly established the immuno-suppression in pregnant goats and damage to immune system by Johne's disease. The study is maiden attempt and reports high presence and recovery of MAP in vaginal secretions of post parturient farm goats in India by culture and ELISA kit. Positive IS900 PCR in DNA pellets confirmed the presence of MAP. Vaginal secretion was potential source of contamination to newborn kids and animal handlers and was a good clinical material to screen post-parturient female goats against MAP infection