Eighth International Colloquium on Paratuberculosis

Session 5: Diagnosis

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Title Presentation Author(s)
MGIT, liquid culture system for detection of MAP in bovine fecal samples [abstract] Keynote Fyock TL, Sweeney RW, Whitlock RH.
Interleukin-12 potentiation of the interferon-gamma test rescues day-old blood samples for diagnosis of paratuberculosis PPD specific cellular mediated immune responses [abstract] Keynote Jungersen G, Grell SN, Clemensen A, Howard CJ.
Evaluation of environmental sampling to determine distribution and herd infection status for Mycobacterium avium subspecies paratuberculosis [abstract] Oral Lombard JE, Smith RL, Wagner BA, McCluskey BJ.
Serodiagnostic markers for Johne's infection and disease in red deer (Cervus elaphus) [abstract] Oral Griffin JFT, Mackintosh CG, Rodgers CR, Liggett S, Cooper M, Spittle E.
Genomic polymorphisms for diagnostics of Mycobacterium avium subspecies paratuberculosis [abstract] Oral Semret M, Alexander DC, de Haas P, Overduin P, van Soolingen D, Cousins D, Behr MA.
Identification and characterization of novel antigens of M. paratuberculosis [abstract] Oral Willemsen PTJ, Westerveen JF, Dinkla A, Bakker D, Kant A, Thole JER.
Longitudinal study of ELISA seroreactivity to Mycobacterium avium subsp. paratuberculosis in infected cattle and uninfected herd mates [abstract] Oral Sweeney RW, Whitlock RH, McAdams S.
Evaluation of three PCR assays for identification of Mycobacterium avium subsp. paratuberculosis from liquid culture [abstract] Poster Anklam KS, Manning EJB, Sreevatsan S, Collins MT.
Optimisation of the interferon - gamma assay to maximise responses to M. ptb. antigen in sheep [abstract] Poster Bosward KL, Begg D, de Silva K, Di Fiore L, Taylor D, Emery D, Whittington RJ.
Application of nested PCR to bodily fluids for the detection of cattle infected with Mycobacterium avium subspecies paratuberculosis (Map) [abstract] Poster Buergelt CD, Williams JE, Moniff G.
Validation of Sequential Mycobacterium avium subspecies paratuberculosis Serology (SMAPS) [abstract] Poster Böttcher J, Hoffmann D, Gangl A, Krabisch P, Klees S, Meier N, Wittkowski G.
Value of bulk-milk-serology for control of Johne's disease [abstract] Poster Böttcher J, Gangl A.
Comparison of two methods for the detection of paratuberculosis in dairy cows in Israel: milk and serum ELISA [abstract] Poster Chaffer M, Friedman S, Koren O, Freed M, Beider Z, Ezra E, Elad D.
Evaluation of five antibody detection tests for bovine paratuberculosis [abstract] Poster Collins MT, Wells SJ, Petrini KR, Collins JE, Schultz RD, Whitlock RH.
Evaluation of real time PCR of pooled fecal samples for detection of Mycobacterium paratuberculosis in cattle [abstract] Poster Couquet C, Chatonnet S, Fremont A, Rebeyroles L, Versmisse Y, Blanchard B.
Impact of Corynebacterium pseudotuberculosis infection on serologic surveillance for Johne's disease in goats [abstract] Poster Cushing HF, Wolf C, Hietala S, Manning EJB.
Identification of Mycobacterium avium subsp. paratuberculosis in tissue and faecal material of sheep by PCR. Comparison with serological data [abstract] Poster De Grossi L, Di Donato F, Micarelli G, Cersini A, Sezzi E.
Direct DNA extraction coupled with culture increases the sensitivity of M. a. paratuberculosis detection [abstract] Poster de Juan L, Álvarez J, Bezos J, Aranaz A, Romero B, Mateos A, Domínguez L.
Detection of Mycobacterium avium subsp. paratuberculosis from goats by Sequence Capture PCR [abstract] Poster Djønne B, Olsen I, Storset A, Valheim M, Nilsen S, Haldorsdottir, Holstad G.
Predictive value of serological techniques in the detection of culled cattle in paratuberculosis affected herds [abstract] Poster González J, Pérez V, Reyes LE, Juste RA, García Marín JF.
Validation of a commercial ELISA kit for assessment of the humoral immune response in Mycobacterium paratuberculosis infected sheep and goats [abstract] Poster Gumber S, Eamens G, Whittington RJ.
Real-time PCR-systems for confirming direct detection of Mycobacterium avium subsp. paratuberculosis [abstract] Poster Herthnek D, Bölske G.
Detection of Mycobacterium avium subsp. paratuberculosis in bovine semen by real-time PCR [abstract] Poster Herthnek D, Englund S, Willemsen PTJ, Bölske G.
Effects of vaccination on Johne's Disease AGID and ELISA tests in experimentally challenged baby goats [abstract] Poster Hines II ME, Pence M, Baldwin C, Whittington L, Musgrove J.
Comparison of the diagnostic value of the Complement Fixation Test (CFT), Polymerase Chain Reaction (PCR) and the culture methods for detecting Mycobacterium avium subsp. paratuberculosis in sheep and goats [abstract] Poster Ikonomopoulos J, Kantzoura B, Fragiadaki E, Pavlik I, Bartos M, Loukas JC, Gazouli M.
The detection of Mycobacterium avium subsp. paratuberculosis in bovine manure using Whatman FTA® card technology and Lightcycler real time PCR [abstract] Poster Jaravata CV, Smith WL, Rensen GJ, Ruzante JM, Cullor JS.
Evaluation of fecal culture pooling methods for detection of Mycobacterium avium subspecies paratuberculosis in a beef herd [abstract] Poster Jensen SM, Lombard JE, Garry FB.
Validation of fecal culture method and a serum ELISA for the diagnosis of paratuberculosis in sheep and goats [abstract] Poster Kostoulas P, Leontides L, Billinis C, Enøe C, Florou M, Sofia M.
Sensitivity and specificity of a commercial Johne's disease ELISA in dairy herds of southern Chile [abstract] Poster Kruze J, van Schaik G, Pradenas M, Haro F, Mella A.
Validation of commercially available ELISA tests for the serodiagnosis of paratuberculosis in Germany [abstract] Poster Köhler H, Burkert B, Pavlik I, Moser I, Möbius P, Martin G.
Interference of anti - M. bovis antibodies in serological tests for paratuberculosis [abstract] Poster Marassi CD, Fraguas S, Gonzaga JS, Ristow P, Ferreira R, Oelemann WMR, Fonseca LS, Lilenbaum W.
Improvement of an in-house ELISA for bovine paratuberculosis serology in Brazil [abstract] Poster Marassi CD, Fonseca LS, Ferreira R, Lilenbaum W, Oelemann WMR.
Development of a luciferase reporter phage assay for identification of Mycobacterium avium subsp. paratuberculosis [abstract] Poster McCusker SE, Mutharia LM.
Agreement of three ELISAs for Johne's Disease [abstract] Poster McKenna SLB, Barkema HW, Keefe GP, Sockett DC.
Test performance evaluation of three ELISAs for Mycobacterium avium subsp. paratuberculosis using tissue and fecal culture as comparison standards [abstract] Poster McKenna SLB, Keefe GP, Barkema HW, Sockett DC.
Comparison of a gel diffusion test, three absorbed ELISAs, a faeces-PCR kit and faecal culture for diagnosis of caprine paratuberculosis in France [abstract] Poster Mercier P, Martin J, Bertin C, Courtes C, Durand B, Boschiroli ML, Laroucau K.
Application of different methods for the diagnosis of experimental paratuberculosis in goats [abstract] Poster Munjal SK, Tripathi BN, Paliwal OP, Boehmer J, Homuth M.
Isolation of atypical mycobacteria from the environment in cattle herds with high and low seroprevalence to Mycobacterium avium subsp. paratuberculosis [abstract] Poster Norby B, Fosgate GT, Roussel AJ, Manning EJB, Collins MT.
Using real-time PCR to solve mycobacterial problems [abstract] Poster O'Mahony J, Hill C.
Contribution of atypical mycobacteria to false-positive reactions to serum ELISA test for paratuberculosis [abstract] Poster Osterstock JB, Roussel AJ, Fosgate GT, Norby B, Manning EJB, Collins MT.
Application of different methods for the detection of M. avium subsp. paratuberculosis (Map) in Argentina [abstract] Poster Paolicchi F, Zumárraga MJ, Gioffré A, Etchechoury I, Morsella C, Cirone K, Cataldi A, Romano MI.
Comparative study between the Johne's absorbed ELISA and complemement fixation test for diagnosis of bovine paratuberculosis [abstract] Poster Petriceanu G, Radulescu RA, Răgălie A.
Validity and costs of pooled fecal culture to detect Mycobacterium avium subsp. paratuberculosis in dairy cows in southern Chile [abstract] Poster Pradenas M, Kruze J, van Schaik G.
Comparative evaluation of the results from the surveillance and confirmation methods applied in ruminants' paratuberculosis [abstract] Poster Radulescu RA, Petriceanu G, Ragalie A, Popescu S, Ion S, Niculae A.
Detecting CMI responses to Mycobacterium avium ssp. paratuberculosis infection in calves born to naturally infected dams [abstract] Poster Robbe-Austerman S, Young S, O'Connor A, Palmer MV, Stabel J.
Comparing the probability of MAP tissue culture positivity in CMI negative and CMI positive sheep [abstract] Poster Robbe-Austerman S, Stabel J, Palmer MV, O'Connor A.
Association of atypical mycobacteria with positive ELISA results for paratuberculosis [abstract] Poster Roussel AJ, Fosgate G, Manning EJB, Collins MT.
Application of a rapid DNA extraction method with real-time fluorescence PCR for the detection of Mycobacterium avium subsp. paratuberculosis in raw milk [abstract] Poster Ruzante JM, Smith WL, Jaravata CV, Gardner IA, Cullor JS.
Comparison of ELISAs on milk and serum for the detection of paratuberculosis in dairy goats [abstract] Poster Salgado M, Kruze J, Collins MT.
Isolation, PCR identification and Real-time quantification of M. paratuberculosis [abstract] Poster Schlederer T, Raberger B.
Development and evaluationof a Real Time-PCR-method for the detection of Mycobacterium avium ssp. paratuberculosis [abstract] Poster Schönenbrücher H, Abdulmawjood A, Bülte M.
Genomic comparisons of MAP strains used to produce Johnin [abstract] Poster Semret M, Olsen I, Smart N, Behr MA.
Comparative evaluation of culture, ELISA and PCR in screening of milk samples for the detection of Mycobacterium avium subsp. paratuberculosis infection in lactating goats [abstract] Poster Singh SV, Kumar S, Singh AV, Sevilla I, Juste RA, Gupta VK.
Differential expression of genes encoding CD30L and P-selectin in cattle with Johne's disease: Progress toward a diagnostic gene expression signature [abstract] Poster Skovgaard K, Pudrith CB, Grell SN, Heegaard PMH, Jungersen G, Coussens PM.
Testing for Mycobacterium avium subspecies paratuberculosis infection in asymptomatic milk herd [abstract] Poster Szteyn J, Wiszniewska A.
Detection of Mycobacterium avium subsp. paratuberculosis IS900 in lesions of bovine paratuberculosis by a supersensitive in situ hybridization technique [abstract] Poster Tanaka S, Kai T, Yamada N, Kawasaki K, Sato M.
A light cycler based real-time PCR assay for the detection of Mycobacterium avium subsp. paratuberculosis in food samples [abstract] Poster Tasara T, Stephan R.
Comparison of BACTEC and MGIT systems for detection of M. paratuberculosis [abstract] Poster Thomas G, Manning EJB, Collins MT.
Detection of Mycobacterium avium subsp. paratuberculosis in formalin- fixed, paraffin embedded tissues of goats by IS900 PCR [abstract] Poster Tripathi BN, Stevenson K.
Tissue PCR and its comparison with other tests for the diagnosis of natural paratuberculosis in goats [abstract] Poster Tripathi BN, Sivakumar P, Paliwal OP, Singh N.
Validation of the Pourquier ELISA to detect antibodies against Mycobacterium avium subspecies paratuberculosis in individual milk samples and bulk milk samples of Dutch dairy cows and herds [abstract] Poster van Maanen C, van Schaik G, van der Meulen A, Waal M, Franken P.
Field validation of the Pourquier ELISA to detect fecal shedding of Mycobacterium avium subspecies paratuberculosis in Dutch dairy cows [abstract] Poster van Schaik G, van Maanen C, Franken P.
Understanding bovine paratuberculosis in beef herds: Testing strategies and potential outcomes [abstract] Poster Villarino MA, Wikse SE, Jordan ER.
Large scale detection of Mycobacterium paratuberculosis (M.ptb) in faecal samples using immunomagnetic capture and TaqMan PCR [abstract] Poster Willemsen PTJ, Ruuls R, Damman M, Bakker D.

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