Kids (0 to 6 months) from organized and farmer's herds were screened by culture and PCR (fecal and tissues) and plate ELISA (serum). Of 50 fecal samples, 56.0% kids developed colonies. Whereas, direct microscopy revealed only 38.0% kids infected with MAP. Screening of 60 kids tissues from slaughterhouse by culture showed 58.3% positive. (Organized herds- 18.1% and farmer's 67.3%). Of 60 tissues MLN (38.3%) and small intestine (43.6%) were positive. However, direct microscopy revealed only 31.6% kids positive. Typical MAP colonies usually appeared around 75 days of inoculation in most of the fecal samples, while in case of tissue samples the maximum number of colonies was observed between 60-90 days post inoculation. Serum samples (334) collected from kids belong to organized and farmers' herds (slaughter house). Screenings of 179 serum samples of kids from organized herds 58.6% were positive in plate ELISA using MAP (bison type) antigen. Whereas, screening of 155 kids from farmer's herds, 35.4% kids were positive. While using MAP bovine type antigen none of the kids could be detected as positive. MAP culture were identified on the basis of primary and molecular characterization using specific IS900 and IS1311 PCR and REA analysis and showed that MAP isolates were bison type. Early lesions were also seen on the histo-pathological examination of some of the tissues.