| Title |
Evaluation of a LAM-based ELISA for the detection of subclinically paratuberculosis-infected cattle |
| Author(s) |
Dekker C1,
Rehm T2,
Koets AP3,
vom Schloss A,
Leuven G4,
Strutzberg K5,
Homuth M5.
|
| Institution(s) |
1 SVANOVA Biotech, Uppsala, Sweden, 2 Department of Microbiology and Infectious Diseases, School of Veterinary Medicine Hannover, Germany, 3 Institute of Infectious Diseases and Immunology, Utrecht University, The Netherlands, 4 Staatliches Veterinäruntersuchungsamt Krefeld, Germany, 5IVD GmbH, School of Veterinary Medicine Hannover, Germany
|
| Source |
Sixth International Colloquium on Paratuberculosis
|
| Section |
4:
Diagnostic Applications And Approaches
|
| Abstract |
An ELISA, using M. paratuberculosis - lipoarabinomannan (LAM) as antigen was evaluated by comparing serological (several ELISAs, CFT) and cultural methods (culture of faeces and ileocaecal lymphnodes) in herds with and without a paratuberculosis history in several areas of Germany and the Netherlands. The LAM-ELISA showed a high discriminatory efficacy and reproducibility. The positive and negative predictive value in infected herds were 76 resp. 85 % with respect to a gold standard (culture of ileocaecal lymphnode). This ELISA was used in an eradication-program in Northern Germany. In combination with faecal culture and hygienic measures an effective decrease of seroprevalence in the herds could be achieved.
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