| Title |
Paratuberculosis in Goats: Comparison between Serological Diagnosis by AGID and ELISA tests. |
| Author(s) |
Vélez-Hernández M1,
Chávez-Gris G1,
Collins MT2,
Suárez Güemes F1.
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| Institution(s) |
1 Facultad de Medicina Veterinaria y Zootecnia, Universidad Nacional Autonoma de Mexico. Circuito Exterior s/no., Ciudad Universitaria. Coyoacan 04510, Mexico City. 2 Department of Pathobiological Sciences, School of Veterinary Medicine, University of Wisconsin, Madison.
|
| Source |
Sixth International Colloquium on Paratuberculosis
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| Section |
4:
Diagnostic Applications And Approaches
|
| Abstract |
In order to evaluate the behaviour of the AGID and the ELISA tests, for the diagnosis of caprine paratuberculosis, 5 serum sampling were performed on 3 naturally infected caprine herds. In both serological tests the PPA-3 antigen was employed. The AGID test detected goats with and without clinical signs. Beside, the ELISA seemed to require lower concentration of antibodies to trigger a positive result. Significative differences (P<0.01) were observed in the prevalences estimated by the AGID and ELISA tests along the 5 serum sampling. The AGID test shows as an useful serological test able to detect clinical such as subclinical Map infected goats. The behaviour of both tests were mostly the same and seemed not to be affected by the different paratuberculosis prevalences estimated on each flock.
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