| Title |
Isolation and Sequencing of Insertion Element IS1311 from Mycobacterium paratuberculosis. |
| Author(s) |
Lambeth M1,
Griffin F1,
Crawford A2,
Mackintosh C3.
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| Institution(s) |
1 Disease Research Lab., Department of Microbiology, University of Otago. 2 AgResearch Molecular Biology Unit, Department of Biochemistry, University of Otago. 3 AgResearch, Invermay Agricultural Centre, Private Bag 50034, Mosgiel, New Zealand.
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| Source |
Sixth International Colloquium on Paratuberculosis
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| Section |
4:
Diagnostic Applications And Approaches
|
| Abstract |
The mycobacterial family of insertion elements include the species specific elements IS61101 and IS9002, which are used as identification tools for M.tuberculosis complex and M. paratuberculosis respectively. The M. avium elements of this family, IS1311 and IS1245, have been used for strain differentiation3. Collins et al4 have recently described the presence of IS1311 in M. paratuberculosis through Southern hybridisation. Here we describe a screening strategy for a M. paratuberculosis genomic library resulting in the isolation of a clone bearing IS1311. Sequencing of this clone has revealed an insertion element with very high homology to the M. avium sequence.
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