| Title |
Sequences of Multi-PCR Products in Variants of Mycobacterium avium subsp. paratuberculosis. |
| Author(s) |
Nishimori K,
Uchida I,
Eguchi M1,
Tanaka K,
Tachibana S2,
Nakaoka Y3,
Nishimori T,
Imai K.
|
| Institution(s) |
Hokkaido Research Station, National Institute of Animal Health, Hitsujigaoka-4, Toyohira, Sapporo, 062-0045 , 1 National Institute of Animal Health, Kannondai-3-1-1, Tsukuba, 305-0856, 2 Ishikari Livestock Hygiene Service Center, Hitsujigaoka-3, Toyohira, Sapporo, 062-0045,and 3 Tokachi Livestock Hygiene Service Center, Kisen 59-6, Kawanishi, Obihiro, 089-11, Japan.
|
| Source |
Sixth International Colloquium on Paratuberculosis
|
| Section |
4:
Diagnostic Applications And Approaches
|
| Abstract |
Six isolates of Mycobacterium avium subsp. paratuberculosis from 1992 to 1995 possessed multi-PCR products (229bp and the higher molecular products), using primer 1 (139-162) and primer 2 (313-367), common IS900-detecting primer pairs. The sequences of the higher molecular products revealed that there was the 55bp-direct repeat from 139 to 193 of the IS900 element upstream of it. The PCR profile of the variants was the mixture of both the profile of the template DNA from the cloning vector with the 229bp-band and that with the higher band. It was speculated that there were 2 kinds of insertion of IS900 in the variants.
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