| Title |
A New Assessment of the Absorbed ELISA for Diagnosis of Sheep Paratuberculosis |
| Author(s) |
Garrido JM1,
Aduriz G,
García Marín JF2,
Pérez V2,
Corpa JM,
Juste RA1.
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| Institution(s) |
1 NEIKER (Instituto Vasco de Investigación y Desarrollo Agrario) (SIMA(. Berreaga, 1. 48160 Derio, Bizkaia. SPAIN 2 Facultad de Veterinaria, Universidad de León, Campus de Vegazana s/n, 24007 León. SPAIN
|
| Source |
Sixth International Colloquium on Paratuberculosis
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| Section |
4:
Diagnostic Applications And Approaches
|
| Abstract |
Diagnosis of sheep paratuberculosis is difficult because of the low efficiency and high costs of culture and inconvenience of serological methods: lack of sensitivity of AGID, and excess of nonspecific reactions in the ELISA. In order to reduce the background noise in the absorbed ELISA we assayed a series of modifications in the protocol essentially based in the use of different conjugates. Three sera of different levels of reaction intensity were used to compare the signal to noise ratio of different conjugates at different concentrations. Once the best combination was chosen, the protocol was tested on a sample of 118 sheep of known paratuberculosis status. When only culture results were considered, the modified ELISA showed a sensitivity of 100%, and an specificity of 77.0%. If only histopathological results were taken as the reference the specificity was 89.1% and the sensitivity 79.2%. When a combination of both techniques was used as the gold standard, sensitivity and specificity reached 96.2% and 100%, respectively.
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