Title Complete nucleotide sequence of a gene encoding the bacterioferritin subunit of Mycobacterium avium subspecies silvaticum.
Author(s) Inglis NF, Stevenson K, Hosie AHF, Sharp JM.
Institution(s) United Kingdom
Source Fourth International Colloquium on Paratuberculosis
Section 4: Biology of M. avium/paratuberculosis
Abstract
A gene encoding the bacterioferritin of M. avium ss. silvaticum has been cloned, sequenced and expressed. Translation of a 477 bp open reading frame revealed a deduced sequence of 159 amino acid residues which was shown to share up to 92% homology with the bacterioferritins of five bacterial genera. The recombinant bacterioferritin exhibits serological cross-reactivity with M. avium ss. paratuberculosis and M. avium ss. silvaticum and a protein of 20-22 kDa in sonicates of M. leprae.

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