| Title |
Culture techniques and media constituents for the isolation of M. paratuberculosis from bovine fecal samples. |
| Author(s) |
Whitlock RH1,
Rosenberger AE1,
Sweeney RW1,
Hutchinson LJ2.
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| Institution(s) |
1New Bolton Center, School of Veterinary Medicine, University of Pennsylvania, Kennett Square, PA 19348, USA and 2Dept. of Veterinary Science, Penn State University, University Park, PA 16802, USA.
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| Source |
Third International Colloquium on Paratuberculosis
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| Section |
1:
Laboratory Diagnosis
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| Abstract |
A variety of techniques and concentrations of media constitutents in Herrold's egg yolk media were evaluated to assess their influence on the recovery of M. paratuberculosis from bovine fecal samples. Sodium pyruvate (4.1 gms/L) enhanced both growth and recovery of M. paratuberculosis compared to media without pyruvate. An increase of 20% egg yolk per liter of media improved M. paratuberculosis recovery. Centrifugation at more than 900 G for thirty minutes did not seem to increase the recovery rate. Increasing the sample size from one (1) to two (2) grams had a major influence, but further increases in sample size had only a minor influence. A 50% increase in Mycobactin-J did not increase recovery while a reduction (50%) in malachite green increased bacterial contamination while decreasing recovery of M. paratuberculosis. Fecal samples mixed with a commercial lubricant did not reduce recovery. Storage of fecal samples at room temperature, 4°C or -20°C did not reduce recovery of M. paratuberculosis when stored in these conditions for up to one week.
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