This investigation was conducted to evaluate the efficiency of conventional old method with that of the new method (two step germination) in our diagnositic laboratory using 5.0 g samples for fecal culture. Using the old method, out of a total of 417 suspected clinical fecal samples cultured, 170 (40.8%) were positive with a contamination rate of 4.96%. During the same period, out of 625 herd samples, 17 (2.7%) were found positive with a 7.2% contamination rate. Using the two step germination procedure, out of a total of 335 suspected clinical fecal samples, 135 (40.29%) yielded positive culture with a contamination rate of 2.08%. Whereas, out of 450 herd samples, 10 (2.22%) were found positive. Furthermore, the same 69 clinical fecal samples were compared in parallel cultures using both methods, of which 26 (37.68%) and 27 (39.1%) were positive by the old and new methods, respectively, indicating a higher sensitivity of the new method. In addition, the new method was considerably superior in reducing the rate of culture contamination.