| Title |
Identification by PCR of M. paratuberculosis in long term cultures from Crohn's disease tissue |
| Author(s) |
Moss M,
Sanderson J,
Tizard M,
Hermon-Taylor J,
El-Zaatari F,
Markesich D,
Graham D.
|
| Institution(s) |
Department of Surgery, St. George's Hospital Medical School, UK. Digestive Disease Section, VAMC and Baylor College of Medicine, Houston, Texas.
|
| Source |
Third International Colloquium on Paratuberculosis
|
| Section |
3:
Related infections
|
| Abstract |
Isolation of M. paratuberculosis from a small number of Crohn's disease (CD) patients has led to renewed interest in the potential role of this organism in the pathogenesis of CD. M. paratuberculosis grows extremely slowly in vitro and yields are invariably low. PCR, however, is ideal for the detection of small numbers of bacilli. Centrifugal pellets from cultures of intestinal tissue from 18 CD patients and 11 non-CD controls were tested for the presence of M. paratuberculosis DNA using a PCR assay based on the 5' region of IS900. In 6 of the 18 CD's and 1 of the 11 controls M. paratuberculosis was positively identified. The intensity of the PCR signal in the positive CD samples was consistent with an abundance of M. paratuberculosis approximating 5-30 genomes per culture pellet. The results are consistent with the presence of M. paratuberculosis in intestinal tissue in CD, in a form which hardly replicates in culture.
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