| Title |
Evaluation of three diagnostic DNA probes tests for Johne's disease on two dairy farms in the Netherlands |
| Author(s) |
van der Giessen J1,
Haring R1,
Eger T2,
Haagsma J2,
van der Zeijst B1.
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| Institution(s) |
1 Institute of Infectious Diseases and Immunology, University of Utrecht and 2Central Veterinary Institute, Lelystad, The Netherlands.
|
| Source |
Third International Colloquium on Paratuberculosis
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| Section |
1:
Laboratory Diagnosis
|
| Abstract |
A DNA probe specific for the 16S ribosomal gene of M. paratuberculosis was developed. This probe was used in a polymerase chain reaction (PCR) based assay for detection of M. paratuberculosis in fecal material of infected cattle. Comparison with the results of culture show that there were no false positive results. However, the sensitivity was considerably lower. A field evaluation was carried out on two dairy herds with a long history of Johne's disease. Two other DNA probe tests were also included in this evaluation. One of these tests used the same fecal preparation method, but the PCR primers were derived from the M. paratuberculosis specific IS900 insertion element. The third test was the commercially available IDEXX M. paratuberculosis DNA probe test. The sensitivity of the 3 DNA probe tests ranged from 13-234% compared to culture results, The specificity of these tests under field conditions was 100%. We conclude that DNA probe tests give rapid and specific results. More research has to be done to increase the sensitivity.
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