Objectives

Establish by RFLP genetic differences of MAP between caprine and ovine isolates, as well as between different geographic areas in several states from Mexico.

Material and Methods

Small ruminants with clinical signs of paratuberculosis were detected using ELISA and AGID tests, necropsy of 51 positive animals were performed and samples of animals with multibacilar lesions were collected. Mycobacteria from freezed intestinal samples were concentrated to obtain DNA. RFLP was performed employing BstEII enzyme and IS900 as probe. Cultures from these samples were developed in Löwenstein Jensen medium.

Results

Serum samples were obtained and 13.14% (137/1043) were positive to serological test. 43.14% (22/51) of the animals included in the anatomopathological study presented multibacilar lesions. Enough DNA for RFLP was obtained only from 12 samples. Two new profiles were identified (C33 and I3) in six ovines. MAP DNA obtained from goat intestinal samples were identified as C type. Types C and I were identified in the ovine origin samples. In the different geographic areas, the types most commonly found were C1 and the new type I3. From all the samples of animals with multibacilar lesions, MAP culture was obtained.