In large paratuberculosis control program, requiring the screening of more than 15.000 cows per year, such as in Israel, bleeding cows for the serum ELISA test, becomes costly and demanding. In the last years, milk ELISA has been developed to check the disease. Milk recording is performed monthly in Israel. More than 100.000 cows, about 88% of the cows in the country, are checked monthly through the milk to which a preservative is added. Examining the same samples for Mycobacterium avium subsp. paratuberculosis (MAP) could significantly reduce the resources needed for the control program. In this study, a commercial milk ELISA for detection of antibodies against MAP was evaluated using milk and preserved milk. In the first stage blood and composite milk was collected from 735 milking cows from four farms. In the second stage blood was collected from 987 milking cows from five farms. Milk was collected on the Milk Recording test day, by the Central Milk Laboratory representative and preserved with bronopol. Serum and milk were tested, according to the manufacturer's instructions, by commercial kits: IDEXX and Pourquier, respectively. Fecal samples from positive cows were cultured and part of these results is pending. The level of agreement between results for serum and milk samples was determined by the kappa statistic.The agreement between the 2 tests assessed using Kappa value was excellent (0.82) when comparing composite milk with serum. Comparison between serum and preserved milk showed that 43 cows were positive in both tests, 6 in serum ELISA and 15 with milk ELISA, the rest of 923 cows were negative in both. The agreement between the tests was good (0.80)Composite milk or preserved milk from the National Milk Recording seems to be a good alternative for serodiagnosis of paratuberculosis in cows.