| Title |
Molecular typing of Mycobacterium avium subsp. paratuberculosis strains from different hosts and regions |
| Author(s) |
Sevilla I1,
Singh SV2,
Garrido JM1,
Aduriz G1,
Rodríguez S3,
Geijo MV1,
Whittington RJ4,
Saunders V5,
Whitlock RH6,
Juste RA1.
|
| Institution(s) |
1Instituto Vasco de Investigación y Desarrollo Agrario (NEIKER). Berreaga, 1. 48160 Derio. Bizkaia, Spain; 2Goat Health Division, Central Institute for Research on goats. Makhdoom. Post Farah Dist. Mathura, (UP) 28 11 22 India; 3Universidad de Córdoba. Facultad de Veterinaria. Campus Universitario de Rabanales. Ctra. Madrid-Cádiz, km. 396. 14071 Córdoba, Spain; 4Faculty of Veterinary Science, University of Sydney, NSW 2006, Australia; 5NSW Agriculture, Camden NSW 2570, Australia; 6USDA-ARS-National Animal Disease Center. New Bolton Center. Kennett Square, PA 19348-1692. USA
|
| Source |
Eighth International Colloquium on Paratuberculosis
|
| Section |
4:
Molecular biology, Microbiology and Culture
|
| Presentation |
Poster
|
| Abstract |
Genotypic differences have been used to characterise isolates of Mycobacterium avium subsp. paratuberculosis (Map), the agent of paratuberculosis in ruminants. The IS1311 Polymerase Chain Reaction-Restriction Endonuclease Analysis (PCR-REA) is a genetic typing technique that distinguishes between C (cattle), S (sheep) and B (bison) type strains of paratuberculosis. This method was used to detect genetic differences between 381 Map isolates from cattle, sheep, goat and bison, coming from distinct regions of Spain, India and United States. In Spain, all 346 bovine isolates, 4 of 10 caprine isolates (40%) and 1 of 12 ovine isolates (8.3%) were of the C type, whereas the other 11 ovine isolates (91.7%) and 6 caprine isolates (60%) were of the S type. All 5 ovine isolates and 6 caprine isolates from India, as well as all 3 isolates from bison (Bison bison) from the USA, were of the B type. This is the first report on the incidence of B type strains in species other than bison, although multiplex Pulsed Field Gel Electrophoresis (PFGE) and Restricted Fragment Length Polymorphism analysis results indicate that they are not the same strain. These results suggest that there are genetic differences between Map isolates related to geographic and host factors that can have a potential for the epidemiological tracing of new paratuberculosis isolates
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