| Title |
Variable number of tandem repeats (VNTRs) and mycobacterial interspersed repetitive units (MIRU) in the genome of members of Mycobacterium avium complex (MAC) |
| Author(s) |
Romano MI1,*,
Amadio A2,
Bigi F1,
Klepp L1,
Etchechoury I1,
Morsella C3,
Paolicchi F3,
Pavlik I4,
Bartos M4,
Leão SC5,
Cataldi A1.
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| Institution(s) |
1Instituto de Biotecnología, Centro de Investigación en Ciencias Veterinarias, del CICVyA - Instituto Nacional de Tecnología Agropecuaria (INTA). Los Reseros y las Cabañas s/n Castelar (1712), Buenos Aires, Argentina; 2Instituto de Microbiología y Zoología Agrícola del CICVyA - Instituto Nacional de Tecnología Agropecuaria (INTA) Castelar, Buenos Aires, Argentina; 3Grupo de Sanidad Animal EEA-INTA Balcarce, Buenos Aires, Argentina; 4Universidade Federal de São Paulo, Escola Paulista de Medicina, São Paulo, Brazil; 5Veterinary Research Institute, Brno, Czech Republic
|
| Source |
Eighth International Colloquium on Paratuberculosis
|
| Section |
4:
Molecular biology, Microbiology and Culture
|
| Presentation |
Poster
|
| Abstract |
Member of MAC are serious pathogens for humans and animals. The aim of this study was to look for VNTR-MIRU loci in the genome of MAC to type these isolates. In the present study, we identified 26 VNTR-MIRU loci by using Tandem Repeat software: eight of them possessed a structure similar to MIRU, 18 of them were tandem repeat without MIRU structure and designated as VNTR. Most VNTR loci were located within predicted coding regions. Most MIRU loci were intercistronic with their extremities overlapping the termination and initiation codons of their flanking genes. Some of these VNTR-MIRU loci exhibited polymorphism among MAC isolates due to insertion or deletion of whole repeats and/or of nucleotide sequence degeneration. We determined the variability of six VNTR-MIRU loci in 21 M. avium subsp. hominissuis (MAH) and 26 M. avium subsp. paratuberculosis (MAP). The results of VNTR-MIRU typing were compared with those obtained by using RFLP and PRA. The analysis identified 15 different alleles with the combination of six VNTR-MIRU loci in the 21 MAH with 16 different IS1245-RFLP and four different PRA profiles. However, neither with the six VNTR-MIRU loci nor with PRA could MAP with 5 different IS900-RFLP profiles be distinguished. In conclusion, some of the VNTR-MIRU loci identified were useful to differentiate MAH isolates but not the MAP isolates here included. However, there were polymorphism in VNTR-MIRU loci between MAH and MAP genomes, which could be important in the understanding of obvious differences in the pathogenic effect of these mycobacteria.
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