Current vaccines for Johne's disease (JD) are highly problematic. A Mycobacterium avium subspecies paratuberculosis (MAP) vaccine that reduced the rate or eliminated disease or fecal shedding would be useful in control of JD. Efficacy of four vaccine combinations, including cell-wall competent (CWC) alum adjuvant, CWC-QS21 adjuvant, cell-wall deficient (CWD) alum adjuvant and CWD-QS21 adjuvant vaccines at both six and nine month post challenge time periods were evaluated. Baby goats were vaccinated at one and four weeks of age with each vaccine or a sham control vaccine consisting of alum adjuvant. Kids were challenged orally with 6.0 X 10⁹ organisms in four divided doses (1.5 X 10⁶ organisms per dose) using a confirmed goat isolate of MAP. Eighty kids were used with each experimental group consisting of 10 kids and each control group 6 kids. Half of the kids within each group were necropsied at six months post challenge and remaining kids were necropsied at nine months post challenge. Gross and microscopic lesions, as well as, relative number of acid-fast bacilli were evaluated and scored at necropsy. Results indicated all challenged kids had some lesions compatible with JD suggesting none of the vaccines prevented infection. Results suggested that three vaccines (CWC-alum, CWC-QS21 and CWD-QS21) reduced lesion scores resulting in 45.6 - 50.6% reduction in lesion scores at the nine-month period. CWD-alum vaccine resulted in a more severe (+33.5%) lesion score than sham-vaccinated challenged control. Lesion scores increased from the six to nine-month necropsy period in the sham-vaccinated challenged group and CWD-alum vaccinated group, while lesion scores were generally stable with remaining vaccines. Fecal and tissue culture results are not yet complete, but will be available for the presentation.