| Title |
Experimental challenge model for paratuberculosis in red deer |
| Author(s) |
Mackintosh CG1,
de Lisle GW2,
Griffin JFT3.
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| Institution(s) |
1AgResearch Invermay, P O Box 50034, Mosgiel, New Zealand; 2AgResearch, Wallaceville Animal Research Centre, Upper Hutt, New Zealand; 3University of Otago, Microbiology and Immunology Department, Dunedin, New Zealand
|
| Source |
Eighth International Colloquium on Paratuberculosis
|
| Section |
2:
Immunology, pathology and pathogenesis
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| Presentation |
Poster
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| Abstract |
Paratuberculosis is an emerging problem in farmed red deer in New Zealand. Red deer often develop clinical disease as young as 8 months of age and suffer outbreaks involving up to 25% of groups of young animals. In order to study the pathogenesis, epidemiology and immunology of this disease in deer, we have developed an experimental infection model that closely mimics natural disease. It utilises oral challenge of 3 month old deer with approximately 109 live "deer/bovine" strain M. paratuberculosis recovered from the mesenteric lymph nodes of clinically affected yearling deer. In two studies involving a total of 59 young deer, clinical Johne's disease developed in 26% and 31% of deer, 5 to 11 months after challenge. The gross and microscopic appearance of the lesions and the immunological responses were indistinguishable from natural Johne's disease in red deer. This model has subsequently been used in vaccine and epidemiological studies.
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