| Abstract |
In Victoria, in 1996, a voluntary JD test and control program was established by the cattle industry and government. Participating farmers are provided with advice on disease control and an annual test of their adult herd using an absorbed ELISA. The test is used annually on all animals two years and older and positive reactors are culled. Private veterinarians under contract with the government deliver the program. There are over 500 dairy herds enrolled in the program. The components of the program and the progress in 36 herds that had completed 4 or more annual herd tests were reported at the previous colloquium.
There are now nearly 400 herds that have completed 5 or more annual herd tests. The evidence for progress in the program is the decline in ELISA reactor prevalence from 1.7% to 1.0% over 7 years and the decline in clinical case rates from about 0.4% to less than 0.1% However, an analysis of the sensitivity of the commercially available ELISA using reactor and clinical case rates in the program indicates a lower diagnostic sensitivity than previous estimates. In any test-round, the number of ELISA positive animals detected was between 20 and 30% of the total number of animals from that round that became ELISA positive or developed clinical disease at later test rounds. Taking into account the number of reactors that would have occurred in animals that were culled or died, the sensitivity is estimated to be between 10 and 15%. The test is read at a cut-point that yields a very high diagnostic specificity.
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